Although we have not analyzed PneumaCult™ media to be used with murine epithelial cells, you can find publications that report this application.
The following case in point is for planning RNP complexes for 1 response. Modify appropriately depending on the number of reactions expected.
Each of those cells has a role to Perform. Study on how they function and their interactions with other subsets proceeds to expose prospects for novel therapeutic approaches.
Utilizing a P1000 pipette, Carefully triturate BLOs inside the apical chamber somewhere around three instances when circling the tip round the insert. Transfer contents to a 15 mL conical tube.
See facts displaying outstanding ALI culture morphology and better epithelial cell marker expression applying these encouraged Transwell® inserts >
The readings can even be executed prior to the lifestyle is evaluated for endpoint characterizations, like electrophysiology or airway marker expression.
EasySep™ is a fast and simple immunomagnetic cell separation technological innovation that means that you can isolate just about any cell varieties from many different sample sources in as tiny as eight minutes. This movie describes the EasySep™ know-how.
Differentiation into adipocytes, osteoblasts, or chondrocytes with compatible MesenCult™ differentiation systems
Yes, you’ll locate the ways to accomplish an ICC staining on your own epithelial cells cultured with the ALI In this particular protocol. Here is a list of antibodies that may be used for the characterization of airway cultures:
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That is an conclude-place protocol built to dissociate PSC-derived kidney organoids into only one-cell suspension and fixation for downstream move cytometry Assessment.
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